Ultra-precision nano-sensor could detect iron disorders
Chronic iron imbalances 鈥� having either too little or too much iron in the blood 鈥� can result in medical conditions ranging from anaemia and haemochromatosis through to more severe diseases, such as cancer, Parkinson鈥檚 Disease and Alzheimer鈥檚 Disease.聽
Haemochromatosis is one of Australia鈥檚 most common hereditary diseases and the Australian Bureau of Statistics estimates approximately 780,000 people live with anaemia.
School of Biomedical Engineering聽PhD candidate and Sydney Nano Institute student ambassador,聽Pooria Lesani, who is undertaking聽his studies under the supervision of Professor聽 and Dr聽Zufu Lu, has a multipurpose nanoscale bio-probe that allows researchers to precisely monitor iron disorders in cells, tissue, and body fluids as small as 1/1000th of a millimolar[1].
The test is more sensitive and specific than blood testing currently used to detect iron disorders, which begin at very low, cellular level concentrations.
Using novel carbon-based fluorescent bio-nanoprobe technology, the test, which involves non-invasive subcutaneous or intravenous injections, allows for a more accurate disease diagnosis before the onset of symptoms, potentially allowing for the early treatment and prevention of more serious diseases.
鈥淢ore than lives with an iron imbalance, which over time can lead to certain forms of cancer, as well Parkinson鈥檚 Disease and Alzheimer鈥檚 Disease,鈥� said Mr Lesani from the Tissue Engineering and Biomaterials Research Unit聽and the聽.
鈥淐urrent testing methods can be complex and time consuming. To counter this, and to enable the early detection of serious diseases, we have developed a hyper-sensitive and cost-efficient skin testing technique for detecting iron in the body鈥檚 cells and tissue.
Iron disorders can lead to serious health issues such as haemochromatosis, cancer, Parkinson鈥檚 Disease and Alzheimer's Disease. Researchers hope the new test will lead to earlier, more accurate disease diagnosis, before the onset of symptoms. Credit: Pooria Lesani, University of Sydney.
鈥淥ur most recent testing demonstrated a rapid detection of free iron ions with remarkably high sensitivity. Iron could be detected at concentrations in the parts per billion range, a rate ten times smaller than previous nano-probes.
鈥淥ur sensor is multifunctional and could be applied to deep-tissue imaging, involving a small probe that can visualise structure of complex biological tissues and synthetic scaffolds.鈥�
Tested on pig skin, the nanoprobe outperformed current techniques for deep tissue imaging, and rapidly penetrated biological tissue to depths of 280 micrometres and remained detectable at depths of up to 3,000 micrometres 鈥� about three millimetres 鈥� in synthetic tissue.
The team aims to test the nanoprobe in larger animal models, as well as investigate other ways in which it can be used to determine the structure of complex biological tissues.
鈥疻e hope to integrate the nanoprobe into a 鈥渓ab-on-a-chip鈥� sensing system 鈥� a portable, diagnostic blood testing tool which could allow clinicians to remotely monitor their patients鈥� health.
鈥淟ab-on-a-chip systems are relatively simple to operate and require only small blood volume samples from the patient to gain an accurate insight of potential ferric ion disorders in the body, assisting early intervention and prevention of disease,鈥� he said.
The nano-sensors can also be made from agricultural and petrochemical waste products, allowing for low-cost, sustainable manufacturing.
DISCLOSURE:
The authors acknowledge funding from The Australian Research Council, The National Health and Medical Council and The Australian Centre for Microscopy and Microanalysis.
1.听听听听听听Refers to the number of聽聽of聽iron per litre of聽blood.